Part:BBa_K4390037
E. coli codon optimised Lambda cI
Usage and Biology
Lambda cI is a transcriptional repressor which allows Lambda phage to establish and maintain latency after infect E. coli. It regulates the entry of lytic cycle by repressing the lytic promoters (Johnson, A. D. et al., 1979). This Lambda cI sequence was codon optimised for expression in E. coli K12, and was be used in Seamless Enrichment of Ligand-Inducible Sensors (SELIS) as the repressor (d’Oelsnitz, S. et al., 2022). In iGEM22_Edinburgh-UHAS_Ghana SELIS design, Lambda cI will be assembled into plasmid pJUMP29-1A(lacZ) using JUMP assembly (Valenzuela-Ortega M and French C., 2021) and expressed under control of either pbrR, merR or arsR. The expressed Lambda cI will be used to repress CmR gene which generates chloramphenicol resistance in bacteria. For further information please refer to any of SELIS arsR evolution construct, SELIS merR evolution construct, SELIS mutated merR evolution construct and SELIS pbrR evolution construct.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
D'Oelsnitz, S. et al., (2022) Using fungible biosensors to evolve improved alkaloid biosyntheses. Nature chemical biology. 18 (9), 981–989.
Johnson, A. D. et al. (1979) Interactions between DNA-Bound Repressors Govern Regulation by the $\lambda $ Phage Repressor. Proceedings of the National Academy of Sciences - PNAS. 76 (10), 5061–5065.
Valenzuela-Ortega, M. & French, C. (2021) Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology. Synthetic biology (Oxford University Press). 6 (1), ysab003–ysab003.
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